title Serpentine bacteria influence metal translocation and bioconcentration of Brassica juncea and Ricinus communis grown in multi-metal polluted soils
authors Ma, Y; Rajkumar, M; Rocha, I; Oliveira, RS; Freitas, H
author full name Ma, Ying; Rajkumar, Mani; Rocha, Ines; Oliveira, Rui S.; Freitas, Helena
nationality nacional
language English
document type Article
author keywords phytostabilization; plant growth promoting bacteria; serpentine soils; heavy metals; Brassica juncea; Ricinus communis
abstract The aim of this study was to assess the effects of inoculation of rhizosphere or endophytic bacteria (Psychrobacter sp. SRS8 and Pseudomonas sp. A3R3, respectively) isolated from a serpentine environment on the plant growth and the translocation and accumulation of Ni, Zn, and Fe by Brassica juncea and Ricinus communis on a multi-metal polluted serpentine soil (SS). Field collected SS was diluted to 0, 25, 50, and 75% with pristine soil in order to obtain a range of heavy metal concentrations and used in microcosm experiments. Regardless of inoculation with bacteria, the biomass of both plant species decreased with increase of the proportion of SS. Inoculation of plants with bacteria significantly increased the plant biomass and the heavy metal accumulation compared with non-inoculated control in the presence of different proportion of SS, which was attributed to the production of plant growth promoting and/or metal mobilizing metabolites by bacteria. However, SRS8 showed a maximum increase in the biomass of the test plants grown even in the treatment of 75% SS. In turn, A3R3 showed maximum effects on the accumulation of heavy metals in both plants. Regardless of inoculation of bacteria and proportion of SS, both plant species exhibited low values of bioconcentration factor (<1) for Ni and Fe. The inoculation of both bacterial strains significantly increased the translocation factor (TF) of Ni while decreasing the TF of Zn in both plant species. Besides this contrasting effect, the TFs of all metals were <1, indicating that all studied bacteria plant combinations are suitable for phytostabilization. This study demonstrates that the bacterial isolates A3R3 and SRS8 improved the growth of B. juncea and R. communis in SS soils and have a great potential to be used as inoculants in phytostabilization scenarios of multi-metal contaminated soils.
author address [Ma, Ying; Rocha, Ines; Oliveira, Rui S.; Freitas, Helena] Univ Coimbra, Ctr Funct Ecol, Dept Life Sci, P-3000456 Coimbra, Portugal; [Rajkumar, Mani] Cent Univ Tamil Nadu, Dept Life Sci, Thiruvarur, India; [Oliveira, Rui S.] Univ Catolica Portuguesa, Ctr Biotecnol & Quim Fina, Lab Associado Escol Super Biotecnol, Oporto, Portugal; [Oliveira, Rui S.] Polytech Inst Porto, Res Ctr Hlth & Environm, Sch Allied Hlth Sci, Vila Nova De Gaia, Portugal
reprint address Ma, Y (reprint author), Univ Coimbra, Ctr Funct Ecol, Dept Life Sci, Calcada Martim Freitas, P-3000456 Coimbra, Portugal.
e-mail address cathymaying@gmail.com
funding agency and grant number Fundacao para a Giencia e a Tecnologia (FCT) [SFRH/BPD/76028/2011, SFRH/BPD/85008/2012]; Fundo Social Europeu; Department of Biotechnology (DBT), Government of India; PCT [BI-EXPL/AGR-TEC/1204/2013]; National Rinds through FCT [EXPL/AGR-TEC/1204/2013]; Fundo Europeu de Desenvolvimento Regional (FEDER); Eixo I do Programa Operacional Fators de Competitividade (POFC) of QREN [COMPETE: FCOMP-01-0124-FEDER-041572]; PEst-OE/BIA/UI4004/2014
funding text Ying Ma and Rui S. Oliveira wish to acknowledge the support of Fundacao para a Giencia e a Tecnologia (FCT) through the research grants SFRH/BPD/76028/2011 and SFRH/BPD/85008/2012 and Fundo Social Europeu, Mani Rajkumar acknowledges the financial support received in the form of Ramalingaswami re-entry fellowship from Department of Biotechnology (DBT), Government of India. Ines Rocha was supported by the PCT grant BI-EXPL/AGR-TEC/1204/2013. This work was supported by National Rinds through FCT under the project EXPL/AGR-TEC/1204/2013, financed by Fundo Europeu de Desenvolvimento Regional (FEDER), Eixo I do Programa Operacional Fators de Competitividade (POFC) of QREN (COMPETE: FCOMP-01-0124-FEDER-041572), and the project PEst-OE/BIA/UI4004/2014.
cited references Abou-Shanab RAI, 2006, SOIL BIOL BIOCHEM, V38, P2882, DOI 10.1016/j.soilbio.2006.04.045; Ali H, 2013, CHEMOSPHERE, V91, P869, DOI 10.1016/j.chemosphere.2013.01.075; Arshad M, 2007, TRENDS BIOTECHNOL, V25, P356, DOI 10.1016/j.tibtech.2007.05.005; Babu AG, 2013, J HAZARD MATER, V250, P477, DOI 10.1016/j.jhazmat.2013.02.014; Bauddh K, 2012, INT J PHYTOREMEDIAT, V14, P772, DOI 10.1080/15226514.2011.619238; Becerra-Castro C, 2012, J HAZARD MATER, V217, P350, DOI 10.1016/j.jhazmat.2012.03.039; Becerra-Castro C, 2013, APPL ENVIRON MICROB, V79, P5094, DOI 10.1128/AEM.00402-13; Bolan NS, 2011, ADV AGRON, V112, P145, DOI 10.1016/B978-0-12-385538-1.00004-4; Cabello-Conejo MI, 2014, PLANT SOIL, V379, P35, DOI 10.1007/s11104-014-2043-7; Giordani C, 2005, ENVIRON MANAGE, V36, P675, DOI 10.1007/s00267-004-0171-1; Chen ZJ, 2013, J HAZARD MATER, V244, P709, DOI 10.1016/j.jhazmat.2012.10.063; Dell'Amico E, 2008, SOIL BIOL BIOCHEM, V40, P74, DOI 10.1016/j.soilbio.2007.06.024; Desideri D, 2010, MICROCHEM J, V95, P174, DOI 10.1016/j.microc.2009.11.010; Doherty JH, 2008, ENVIRON POLLUT, V151, P593, DOI 10.1016/j.envpol.2007.04.002; Duman F, 2010, J ENVIRON SCI-CHINA, V22, P526, DOI 10.1016/S1001-0742(09)60137-6; Ferraz P, 2012, PLANT PHYSIOL BIOCH, V57, P254, DOI 10.1016/j.plaphy.2012.05.025; Freitas H, 2004, CHEMOSPHERE, V54, P1625, DOI 10.1016/j.chemosphere.2003.09.045; Grandlic CJ, 2008, ENVIRON SCI TECHNOL, V42, P2079, DOI 10.1021/es072013i; He HD, 2013, CHEMOSPHERE, V90, P1960, DOI 10.1016/j.chemosphere.2012.10.057; Israr M, 2011, J HAZARD MATER, V186, P1520, DOI 10.1016/j.jhazmat.2010.12.021; JACKSON AP, 1991, PLANT SOIL, V132, P179; Jebara SH, 2015, ENVIRON SCI POLLUT R, V22, P2537, DOI 10.1007/s11356-014-3510-7; Jiang CY, 2008, CHEMOSPHERE, V72, P157, DOI 10.1016/j.chemosphere.2008.02.006; Kavamura VN, 2010, BIOTECHNOL ADV, V28, P61, DOI 10.1016/j.biotechadv.2009.09.002; Kayama M, 2006, TREES-STRUCT FUNCT, V20, P430, DOI 10.1007/s00468-006-0057-3; Kilburn M., 2000, ANAL METHODS MANUAL, V115; Li KF, 2011, J HAZARD MATER, V189, P531, DOI 10.1016/j.jhazmat.2011.02.075; LINDSAY WL, 1978, SOIL SCI SOC AM J, V42, P421; Lotfy SM, 2014, J GEOCHEM EXPLOR, V144, P367, DOI 10.1016/j.gexplo.2013.07.003; Ma Y, 2011, INT J PHYTOREMEDIAT, V13, P126, DOI 10.1080/15226511003671403; Ma Y, 2013, CHEMOSPHERE, V93, P1386, DOI 10.1016/j.chemosphere.2013.06.077; Ma Y, 2011, BIOTECHNOL ADV, V29, P248, DOI 10.1016/j.biotechadv.2010.12.001; Ma Y, 2009, J HAZARD MATER, V166, P1154, DOI 10.1016/j.jhazmat.2008.12.018; Ma Y, 2009, CHEMOSPHERE, V75, P719, DOI 10.1016/j.chemosphere.2009.01.056; Ma Y, 2011, J HAZARD MATER, V195, P230, DOI 10.1016/j.jhazmat.2011.08.034; Mastretta C, 2009, INT J PHYTOREMEDIAT, V11, P251, DOI 10.1080/15226510802432678; Meers E, 2007, GEODERMA, V141, P247, DOI 10.1016/j.geoderma.2007.06.002; Mengoni A, 2004, MICROBIAL ECOL, V48, P209, DOI 10.1007/s00248-003-0149-1; Niu ZX, 2007, J ENVIRON SCI-CHINA, V19, P961, DOI 10.1016/S1001-0742(07)60158-2; Ouzounidou G, 2006, ARCH ENVIRON CON TOX, V50, P346, DOI 10.1007/s00244-005-5076-3; Perez-Lopez R, 2014, GEODERMA, V230, P194, DOI 10.1016/j.geoderma.2014.04.004; Prapagdee B, 2013, CHEMOSPHERE, V92, P659, DOI 10.1016/j.chemosphere.2013.01.082; Rajkumar M, 2012, BIOTECHNOL ADV, V30, P1562, DOI 10.1016/j.biotechadv.2012.04.011; Rajkumar M, 2013, J ENVIRON MANAGE, V128, P973, DOI 10.1016/j.jenvman.2013.07.001; Rajkumar M, 2013, ENVIRON INT, V53, P74, DOI 10.1016/j.envint.2012.12.009; Rashotte AM, 2000, PLANT PHYSIOL, V122, P481, DOI 10.1104/pp.122.2.481; Rauret G, 1999, J ENVIRON MONITOR, V1, P57, DOI 10.1039/a807854h; Sessitsch A, 2013, SOIL BIOL BIOCHEM, V60, P182, DOI 10.1016/j.soilbio.2013.01.012; Shi X, 2011, J ENVIRON SCI-CHINA, V23, P266, DOI 10.1016/S1001-0742(10)60402-0; Sinegani AAS, 2008, PLANT SOIL ENVIRON, V54, P434; Singh DB, 1996, COLLOID SURFACE A, V111, P49, DOI 10.1016/0927-7757(95)03468-4; Srivastava S, 2013, J HAZARD MATER, V262, P1039, DOI 10.1016/j.jhazmat.2012.08.019; Srivastava S, 2014, ECOL ENG, V70, P146, DOI 10.1016/j.ecoleng.2014.05.008; Testiati E, 2013, J HAZARD MATER, V248, P131, DOI 10.1016/j.jhazmat.2012.12.039; Velikova V, 2011, ENVIRON POLLUT, V159, P1058, DOI 10.1016/j.envpol.2010.10.032; Wang HL, 2004, NEW ZEAL J AGR RES, V47, P389; Wu QH, 2011, INT J PHYTOREMEDIAT, V13, P788, DOI 10.1080/15226514.2010.525562; Zaidi S, 2006, CHEMOSPHERE, V64, P991, DOI 10.1016/j.chemosphere.2005.12.057; Zayed A, 1998, J ENVIRON QUAL, V27, P715; Zhang YF, 2011, CHEMOSPHERE, V83, P57, DOI 10.1016/j.chemosphere.2011.01.041; Zhu LJ, 2014, CHEMOSPHERE, V113, P9, DOI 10.1016/j.chemosphere.2014.03.081
cited reference count 61
publisher city LAUSANNE
publisher address PO BOX 110, LAUSANNE, 1015, SWITZERLAND
issn 1664-462X
29-character source abbreviation FRONT PLANT SCI
iso source abbreviation Front. Plant Sci.
publication date JAN 5
year published 2015
volume 5
article number 757
digital object identifier (doi) 10.3389/fpls.2014.00757
page count 13
web of science category Plant Sciences
subject category Plant Sciences
document delivery number AZ1WA
unique article identifier WOS:000348024500002
CFE authors
Ying Ma