title Contrasting soil fungal communities in Mediterranean pine forests subjected to different wildfire frequencies
authors Buscardo, E; Rodriguez-Echeverria, S; Freitas, H; De Angelis, P; Pereira, JS; Muller, LAH
author full name Buscardo, Erika; Rodriguez-Echeverria, Susana; Freitas, Helena; De Angelis, Paolo; Pereira, Joao Santos; Muller, Ludo A. H.
nationality nacional
source FUNGAL DIVERSITY
language English
document type Article
author keywords 454 Pyrosequencing; DGGE; Wildfire frequency; Soil fungal community; Maritime pine
keywords plus NITROGEN DEPOSITION GRADIENT; GEL-ELECTROPHORESIS ANALYSIS; ALASKAN BOREAL FORESTS; 16S RIBOSOMAL-RNA; ECTOMYCORRHIZAL FUNGI; MICROBIAL-POPULATIONS; LITTER DECOMPOSITION; RESISTANT PROPAGULES; SCLEROPHYLL FOREST; SPATIAL STRUCTURE
abstract Mediterranean forest ecosystems are characterized by various vascular plant groups with their associated mycorrhizae and free living soil fungi with various ecological functions. Fire plays a major role in Mediterranean ecosystem dynamics and impacts both above- and below-ground community structure and functioning. However, studies on the effects induced by altered disturbance regimes (associated with recent land use and climate extremes) on fire ecology and especially on its below-ground impacts are few. The objectives of this study were to evaluate the effects of different wildfire regimes on soil fungal community structure using two different molecular methods. We investigated the long-term effects of wildfire on soil fungal communities associated with Pinus pinaster forests in central Portugal, by comparing the results of denaturing gradient gel electrophoresis (DGGE)-based profiling with those obtained with 454 pyrosequencing. Four forest stands with differing fire history and fire return interval, and vegetation cover (mature forest, early successional stage of pine regeneration, and forest converted to scrubland) were sampled 6 years after the last fire event. The pyrosequencing-based approach indicated ca. eight-fold higher numbers of taxa than DGGE. However, fungal community fingerprinting data obtained for the different study stands with DGGE were congruent with those obtained with pyrosequencing. Both short (7.6 years) and long (24 years) fire return intervals (indicated by the presence of ericaceous shrubs in the understorey) induced a decrease in the abundance ratio between basidiomycetes and ascomycetes and appeared to reduce the frequency of ectomycorrhizal fungal species and saprophytes. Wildfire significantly reduced the frequency of late stage successional taxa (e.g. Atheliaceae and Cantharellales) and known or putative saprophytes belonging to the Clavulinaceae and the Archaeorhizomycetaceae. Conversely, early successional fungal species belonging to the Thelephoraceae were favoured by both fire return intervals, while the abundance of Cortinarius and Hebeloma, which include several Cistus-specific species, increased with short wildfire return intervals. This last finding highlights the relationship between post-fire vegetation composition and cover (vegetation successional stage), and fungal symbionts. We hypothesise that these changes could, in the long term, exhaust the resilience of Mediterranean pine forest vegetation and associated soil fungal communities by preventing pine regeneration.
author address [Buscardo, Erika; Rodriguez-Echeverria, Susana; Freitas, Helena; Muller, Ludo A. H.] Univ Coimbra, Dept Ciencias Vida, Ctr Ecol Func, P-3000456 Coimbra, Portugal; [Buscardo, Erika; De Angelis, Paolo] Univ Tuscia, Dept Innovat Biol Agrofood & Forest Syst DIBAF, I-01100 Viterbo, Italy; [Buscardo, Erika] INPA, Escritorio Cent LBA, BR-69060001 Manaus, Amazonas, Brazil; [Pereira, Joao Santos] Univ Tecn Lisboa, Inst Super Agron, Dept Engn Florestal, P-1349018 Lisbon, Portugal; [Muller, Ludo A. H.] Free Univ Berlin, Inst Biol Bot, D-14195 Berlin, Germany
reprint address Muller, LAH (reprint author), Free Univ Berlin, Inst Biol Bot, Altensteinstr 6, D-14195 Berlin, Germany.
e-mail address ludo.muller@fu-berlin.de
researcherid number De Angelis, Paolo/A-6863-2012
orcid number De Angelis, Paolo/0000-0001-8310-8831
funding agency and grant number Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/21730/2005]
funding text We would like to thank the Associacao de Produtores Florestais e Agricolas of the council of Proenca-a-Nova for providing access to their land. Research was supported by the Portuguese Foundation for Science and Technology (FCT) through a Ph.D. grant for E.B. (SFRH/BD/21730/2005). Figure 1 is courtesy of the Integration and Application Network, University of Maryland Center for Environmental Science (ian.umces.edu/symbols/). We also thank the editor, two anonymous reviewers and Laszlo Nagy for carefully reading the manuscript and for their valuable comments.
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cited reference count 97
publisher SPRINGER
publisher city NEW YORK
publisher address 233 SPRING ST, NEW YORK, NY 10013 USA
issn 1560-2745
29-character source abbreviation FUNGAL DIVERS
iso source abbreviation Fungal Divers.
publication date JAN
year published 2015
volume 70
issue 1
beginning page 85
ending page 99
digital object identifier (doi) 10.1007/s13225-014-0294-5
page count 15
web of science category Mycology
subject category Mycology
document delivery number AZ0TH
unique article identifier WOS:000347957000005
CFE authors
Erika Buscardo
Susana Rodríguez Echeverría